Table 7. Success rates of Second-oocyte collection by cutting the position at the First-oocyte collection.
Cutting position | No. of oviducts | No. (%) * of oviducts contained COCs | No. (average) ** of normal oocytes collected *** |
---|---|---|---|
Control | 12 | 12 (100) a | 199 (16.6) a |
Front | 17 | 13 (76.5) a | 230 (13.5) a |
Middle | 13 | 4 (30.8) b | 66 (5.1) b |
Rear | 40 | 10 (25.0) b | 190 (4.8) b |
COCs: Cumulus oocytes complexes. Total 41 mice were used in this experiment. For some mice, the ampulla was cut at different positions in the same individual.* Different letters indicate significant differences by Pearson’s Chi-square test (P < 0.01). ** Different letters indicate significant differences by t-test (P < 0.01). *** Number of oocytes collected per an oviduct.