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. 2020 Oct 15;10:554331. doi: 10.3389/fonc.2020.554331

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Copyright © 2020 Zhang, Fan, Wang, Yang, Wu, Huang, Lan, Liao, Huang, Yuan, Pan, Wu, Chen and Guan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Structure of the carbohydrate sulfotransferase 4 (CHST4) gene and its distribution in BALB/c mice tissues. (A) Chromosome location (red line; top) and gene structure of CHST4 (bottom; black boxes represent exons and gray boxes represent introns). (B) Relative expression of CHST4 mRNA in various tissues of BALB/c mice by quantitative real-time (qRT)-PCR. GAPDH was used as the loading control. Bars represent the mean ± SD (n = 4); *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test). All samples were tested in triplicate. (C) Immunohistochemical analysis of the expression and distribution of CHST4 protein in BALB/c mice tissues (100×; 400×; four samples in each group).