Table 3.
Normalized (tumor/healthy mucosa) gene expression (nRQ) for all paired samples.
| All samples (n = 13 pairs) | |||||
|---|---|---|---|---|---|
| Mean | Min, Max | Trend in tumor | Correlation with metabolites | p-value | |
| IDO1 (nRQ) | 0.71 | 0.10, 2.41 | = | NAm (r = 0.424) | <0.05 |
| TDO2 (nRQ) | 1.56 | 0.09, 10.04 | ↑ | ||
| Calculated activity | 230 (111) | 8.6, 636 (2.19, 441) | ↑ | KYN (r = 0.803) TRP (r = −0.461) |
<0.0001 <0.05 |
| TPH1 (nRQ) | 0.43 | 0.001, 3.65 | ↓ | 5-HT (r = 0.810) | <0.0001 |
| Calculated activity | 36.3 (40.7) | 1.2, 148 (8.6, 115.7) | = | 5-HTP (r = 0.680) | <0.0001 |
Data are presented as mean fold change and minimum and maximum (Min, Max) values. Enzymatic activity is reported as calculated mean value for rectal cancer samples (healthy mucosa) and relative minimum, maximum. “Trend in tumor” column highlights fold changes at least >1.5 (or <0.67). Correlation between gene expression and TRP metabolites was evaluated using Spearman's rank test and only significant results are reported.