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PLOD1 promotes migration and invasion of osteosarcoma cells. Transwell assay was used to detect the effect of PLOD1 on migration (a–c) and invasion (d–f) of MG-63 and U-2OS cells. (g, h) Western blot was performed to detect expression of MMP-2. (i) IF staining of vimentin in U-2OS cells. GAPDH was used as loading control. NC: normal control. Scan bar, 10 μm. ∗∗∗P < 0.001.
