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. 2020 Oct 15;11:571334. doi: 10.3389/fimmu.2020.571334

Figure 6.

Figure 6

Induction of innate immune signaling pathways in response to C. acnes infection in cGAS-deficient human macrophages. (A) Differentiated wildtype, STING-deficient and cGAS-deficient THP-1 cells were infected with either C. acnes strain NCTC737, L.m. or stimulated with lipopolysaccharide (LPS; 0.4 µg/ml) for either 6 (TNF-α, IL-1β) or 24 h (MX1, IFIT1). (B) Differentiated wildtype, STING-deficient and cGAS-deficient THP-1 cells were infected with either C. acnes strain Asn12, L.m. or stimulated with lipopolysaccharide (LPS; 0.4 µg/ml) for either 6 (TNF-α, IL-1β) or 24 h (MX1, IFIT1). (A, B) HPRT-normalized gene expression was measured using RT-qPCR and shown as log transformed fold change to the uninfected sample. Data represent the mean values of four independent experiments. P values were calculated using the unpaired t-test of log transformed values (*P ≤ 0.05; ***P ≤ 0.001; ****P ≤ 0.0001).