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. 2020 Oct 15;11:584011. doi: 10.3389/fpls.2020.584011

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Copyright © 2020 Burman, Chandran and Khurana.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transient expression of pSITE-2CA (empty GFP vector) construct and BiFc of OsbZIP48 in rice seedling. (A–C) GFP localization of pSITE-2CA vector in rice epidermal cells at 63x. (D–F) GFP localization of pSITE-2CA vector in rice coleoptile cells at 63x. (G–I) GFP localization of pSITE-2CA vector in rice root cells at 63x. Arrow indicates root hair. (J–L) BiFc showing homodimerization of OsbZIP48 (using OsbZIP48 cloned into BiFc vectors pSITE-nEYFP-C1 and pSITE-nEYFP-N1) in nucleus of rice epidermal cells at 63X. (A,D,G,J) Show cells under dark field. (B,E,H,K) Show same cells under bright field with (K) also showing DAPI stained nucleus. Arrow indicates DAPI stained nucleus. (C,F,I,L) Show merged picture of the same cells.