| Chemicals | Amount (for 500 ml) |
| NB medium (Himedia, # PT107) | 12.07 g |
| L-Proline (Duchefa) | 1.435 g |
| L-Glutamine (Duchefa) | 0.25 g |
| Caesin enzyme hydrosylate Type-1 (Himedia) | 0.15 g |
| Sucrose (Qualigens) | 5 g (in addition to 20 g/L already present in commercially available NB medium) |
| D-(+)-Glucose (Sigma) | 10 g |
| Acetosyringone (Himedia) | 30 mg/L (Dissolved in methanol; freshly prepared) |
| Silwet® L-77 (Lehle seeds) | 0.02% v/v |
| Add water to make up the volume and set pH to 5.2 by 1 M KOH | |
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