Chemicals	Stock solution	Working concentration	Amount
Protein extraction buffer			For 10 ml
Tris-Cl buffer (pH 8) (Bio-rad)	1 M	200 mM	2 ml
NaCl (Sigma)	1 M	100 mM	1 ml
Sucrose (Qualigens)	-	400 mM	1.369 g
EDTA, pH 8 (SRL Diagnostics)	1 M	10 mM	100 μl