Chemicals	Stock solution	Working concentration	Amount
DTT (Himedia)	-	10 mM	15.42 mg
Plant protease inhibitor cocktail (Sigma)	-	1%	100 μl (to be freshly added)
Tween 20 (Bio-rad)	-	0.05%	5 μl
PMSF (Sigma)	100 mM	1 mM	100 μl (to be freshly added)
Glycerol (Sigma)	-	5%	500 μl
Milli-Q water			To make up the volume
GUS assay reaction buffer			For 10 ml
EDTA, pH 8 (SRL diagnostics)	1 M	10 mM	100 μl
Sodium dodecyl sulfate (SDS) (Sigma)	10%	0.1%	100 μl
Sodium phosphate buffer, pH 7 (Sigma)	1 M	50 mM	500 μl
Triton X-100 (Sigma)	-	0.1%	10 μl
4-Methylumbelliferyl-β-D-glucuronide hydrate or MUG (Sigma)	-	0.6 mM	2.1 mg
β-Mercaptoethanol (Gibco)	-	12 mM	8.3 μl
Water			To make up the volume
Stop buffer			For 10 ml
Sodium carbonate (Sigma)	-	0.2 M	212 mg in Milli-Q