KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| gp64-PE antibody | Expression Systems | Cat#97-201 |
| monoclonal ANTI-FLAG M2-Peroxidase (HRP) antibody | Sigma-Aldrich | Cat#A8592 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| AEBSF | GoldBio | Cat#A-540-5 |
| Leupeptin | Sigma | Cat#L2884 |
| Aprotinin | GoldBio | Cat#A-655-100 |
| Iodoacetamide | Sigma | Cat#I1149 |
| n-dodecyl-beta-D-Maltopyranoside (DDM) | Anatrace | Cat#D310 |
| Lauryl maltose neopentyl glycol (LMNG) | Anatrace | Cat#NG310 |
| Glyco-Diosgenin (GDN) | Anatrace | Cat#GDN101 |
| GDP | Sigma Aldrich | Cat#M0398L |
| His-tagged PreScission protease | GenScript | Cat#Z0392-500 |
| Tris HCL | VWR | Cat#0497 |
| HEPES | Fisher Scientific | Cat#BP310 |
| Glycerol | Fisher Scientific | Cat#BP229 |
| EDTA | Sigma Aldrich | Cat#E6758 |
| Imidazole | Sigma | Cat#I0250 |
| Sodium Chloride | Fisher | Cat#BP358 |
| Potassium Chloride | Sigma | Cat#793590 |
| Magnesium Chloride | Sigma | Cat#M0250 |
| BSA, Free Fatty Acid | Akron | Cat#AK8909 |
| Ascorbic Acid | Sigma | Cat#A5960 |
| Cholesteryl heisucinate (CHS) | Sigma | Cat#C6512 |
| TALON IMAC resin | Clontech | Cat#635507 |
| 1-Oleoyl-rac-glycerol (monoolein) | Sigma | Cat#M7765 |
| Cholesterol | Sigma | Cat#C8667 |
| Coelenterazine-h | Promega | Cat#S2011 |
| Coelenterazine 400a (Deep Blue C) | Nanolight | Cat#340-1 |
| Poly-L-lysine | Sigma | Cat#P2636 |
| Penicillin/Streptomycin | Invitrogen | Cat#15140-122 |
| Zeocin | Invitrogen | Cat#R25005 |
| Sf-900 II SFM | Invitrogen | Cat#10902096 |
| ESF921 | Expression Systems | Cat#96-001-01 |
| CCM3 media | GE healthcare | Cat#SH3061.04 |
| DMEM | VWR | Cat#45000-306 |
| FBS | VWR | Cat#97068-085 |
| Dialyzed FBS | Omega Scientific | Cat#FB-03 |
| 10xHBSS | Invitrogen | Cat#14065-056 |
| Cellfectin II Reagent | Invitrogen | Cat#10362-100 |
| TransIT-2020 | Mirus | Cat#MIR5400 |
| Polyethylenimine (PEI) | Polysciences, Inc | Cat#23966 |
| [3H]-LSD | Perkin Elmer | Cat#NET638 |
| Methiothepin mesylate salt | Sigma | Cat#M149 |
| Polyethylene glycol 400 | Hampton Research | HR2-603 |
| StockOptions Salt Kit | Hampton Research | HR2-245 |
| Additive Screen HT | Hampton Research | HR2-138 |
| Paraformaldehyde Solution, 4% in PBS | Fisher | Cat#AAJ 19943K2 |
| Critical Commercial Assays | ||
| Bac-to-Bac Baculovirus Expressiokn system | Invitrogen | Cat#A11100 |
| pcDNA 3.1 | Thermo Fisher | Cat#V79020 |
| SuperSignal ELISA Pico Chemiluminescent Substrate | Thermo Fisher | Cat#37070 |
| Deposited Data | ||
| HTR2A/25CN-NBOH/mGq/Gβ/G | This paper | PDB: 6WHA |
| HTR2A/LSD complex structure | This paper | PDB: 6WGT |
| HTR2A/methiothepin complex structure | This paper | PDB: 6WH4 |
| Experimental Models: Cell Lines | ||
| Spodoptera frugiperda Sf9 cells | Expression Systems | Cat#94-001S |
| HEK293T | ATCC | Cat# |
| Recombinant DNA | ||
| Human 5HT2A gene | Integrated DNA Technologies (IDT) | N/A |
| miniGqiN chimera | Integrated DNA Technologies (IDT) | N/A |
| Gβ1 subunit | Integrated DNA Technologies (IDT) | N/A |
| Gγ2 subunit | Integrated DNA Technologies (IDT) | N/A |
| pFastBac dual expression vector | Invitrogen | Cat#10712024 |
| pFastBac 1 expression vector | Invitrogen | Cat#10359016 |
| Software and Algorithms | ||
| COOT | COOT | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot |
| XDS | Kabsch, 2010a, 2010b | http://xds.mpimf-heidelberg.mpg.de/ |
| IUPHAR | Southan et al., 2016 | https://www.guidetopharmacology.org/ |
| Marvin v.15.11.23.0 | ChemAxon | https://chemaxon.com |
| Phaser | McCoy et al., 2007 | https://www.ccp4.ac.uk |
| Phenix | Adams et al., 2010 | https://www.phenix-online.org |
| PyMOL | Schrödinger | https://pymol.org/2/ |
| Prism v8.0 | GraphPad Software | N/A |
| Relion | Scheres, 2012 | https://www3.mrc-lmb.cam.ac.uk/relion/index.php?title=Main_Page |
| SerialEM | Mastronarde, 2003 | https://bio3d.colorado.edu/SerialEM/ |
| REFMAC | Murshudov et al., 1997 | https://www.ccp4.ac.uk |
| MORPHEUS | N/A | https://software.broadinstitute.org/morpheus/ |
| Adobe Illustrator CC | Adobe | https://www.adobe.com |
| Adobe Photoshop CC | Adobe | https://www.adobe.com |
| Other | ||
| PD MiniTrap G-25 columns | GE Healthcare | Cat#28-9180-07 |
| 100 kDa molecular weight cut-off Vivaspin 20 concentrator | Sartorius Stedim | Cat#VS2042 |
| 100 kDa molecular weight cut-off Vivaspin 500 centrifuge concentrator | Sartorius Stedim | Cat#VS0142 |
| 96-well LCP glass sandwich set | Marienfeld GmbH | Cat#0890003 |
| Meltilex | Perkin Elmer | Cat#1450-441 |
| Filtermat A | Perkin Elmer | Cat#1450-421 |
| 96-well black plates | Greiner Bio-one GmbH | Cat#655090 |
| Superdex 200 Increase column | GE healthcare | Cat#289909944 |
| 96-well white plates | Greiner Bio-one GmbH | Cat#655098 |
| Amicon Ultra –4 10K | Merck Millipore | Cat#UFC801024 |
| Amicon Ultra –4 50K | Merck Millipore | Cat#UFC805024 |