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. Author manuscript; available in PMC: 2020 Oct 29.
Published in final edited form as: Cell Rep. 2020 Oct 13;33(2):108248. doi: 10.1016/j.celrep.2020.108248

Figure 4. CBX2 Binds DNA, which Promotes LLPS In Vitro and In Vivo.

Figure 4.

(A) Determination of the binding of CBX2 and CBX2AT-P2A to DNA by EMSA.

(B) Quantification of EMSA gel from (A) to estimate the dissociation constant of CBX2 to DNA.

(C) Example DIC images of CBX2 condensates on the surface of coverslip in the absence or presence of PEG, DNA, or both. Scale bar, 5.0 μm.

(D) Number of condensates quantified from (C). Error bars represent SD.

(E) Example live-cell epifluorescence images of CBX2 and its variants with impaired DNA-binding capacity. Scale bars, 2.0 μm.