Antibodies |
|
|
Anti-HaloTag® pAb |
Promega |
Cat#G9281 |
Anti-RING1B mAb |
MBL Life Science |
Cat#D139–3 |
Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 |
Life Technologies |
Cat#A-11029; RRID: AB_2534088 |
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 |
Life Technologies |
Cat#A11011; RRID: AB_143157 |
Anti-PHC1 antibody (mAb) |
Active Motif |
Cat#39723; RRID: AB_2713961 |
Bacterial and Virus Strains |
|
|
DH5α |
Life Technologies |
Cat#18265–017 |
Rosetta 2 (pLysS) host strains |
Novagen |
Cat#71403 |
Chemicals, Peptides, and Recombinant Proteins |
|
|
ANTI-FLAG® M2 affinity gel |
Millipore |
Cat#A2220 |
Doxycycline hyclate |
Sigma Life Science |
Cat#D9891 |
Fetal Bovine Serum (FBS) |
VWR |
Cat#97068–085 |
FLAG® Peptide |
Sigma-Aldrich |
Cat#F3290 |
FluoroBrite DMEM Live-cell Imaging Medium |
Life Technologies |
Cat#A1896701 |
GSH-Sepharose 4B beads |
GE Healthcare |
Cat#17–--01 |
HaloTag® TMR Ligand |
Promega |
Cat#G8251 |
IPTG |
IBI Scientific |
Cat#IB02105 |
Janelia Fluor® 549 HaloTag® Ligand |
Promega |
Cat#GA1110 |
Janelia Fluor® 646 HaloTag® Ligand |
Promega |
Cat#GA1120 |
Leukemia Inhibitor Factor |
Purified in lab |
N/A |
Polyethylene glycol (PEG) |
Rigauku Reagents |
Cat#1008062 |
SYBR Gold |
Invitrogen |
Cat#S11494 |
Critical Commercial Assays |
|
|
Amicon Ultra Centrifugal Filter Units |
Merck Millipore |
Cat#UFC501024 |
Bradford Assay |
Thermo Scientific |
Cat#1856209 |
CoverWell perfusion chamber gasket |
Thermo Scientific |
Cat#C18139
|
Novex 4–20% Tris-Glycine Protein gel |
Life Technologies |
Cat#EC6026BOX |
NuPAGE 4–12% Bis-Tris Protein Gel |
Invitrogen |
Cat#NP0321BOX |
Spectra/Por 1 Dialysis Membrane |
Spectrum Labs |
Cat#132645T |
Experimental Models: Cell Lines |
|
|
Human: HEK293T |
Tatavosian et al., 2019 |
N/A |
Mouse: PGK12.1: Cbx2−/−
|
Katoh-Fukui et al., 1998 |
N/A |
Mouse: PGK12.1: Eed−/−
|
Endoh et al., 2008 |
N/A |
Mouse: PGK12.1: HT-CBX2/Eed−/−
|
Tatavosian et al., 2019 |
N/A |
Mouse: PGK12.1: HT-CBX2/Mel18−/−/Bm1i−/−
|
Tatavosian et al., 2019 |
N/A |
Mouse: PGK12.1: HT-CBX2 |
Zhen et al., 2016 |
N/A |
Mouse: PGK12.1: HT-CBX2/Ring1a−/−/Ring1bfl/fl; Rosa26::CreERT2
|
Tatavosian et al., 2019 |
N/A |
Mouse: PGK12.1: HT-CBX21−192
|
This study |
N/A |
Mouse: PGK12.1: HT-CBX21−281
|
This study |
N/A |
Mouse: PGK12.1: HT-CBX21−498
|
This study |
N/A |
Mouse: PGK12.1: HT-CBX265−532
|
This study |
N/A |
Mouse: PGK12.1: HT-CBX289−532
|
This study |
N/A |
Mouse: PGK12.1: HT-CBX2ATLm
|
This study |
N/A |
Mouse: PGK12.1: HT-CBX2ATL-P2A
|
This study |
N/A |
Mouse: PGK12.1: HT-CBX2ATm
|
This study |
N/A |
Mouse: PGK12.1: HT-CBX2AT-P2A
|
This study |
N/A |
Mouse: PGK12.1: HT-CBX2CA-P2A
|
This study |
N/A |
Mouse: PGK12.1: HT-CBX2cd
|
This study |
N/A |
Mouse: PGK12.1: HT-CBX2hncr-P2A
|
This study |
N/A |
Mouse: PGK12.1: HT-CBX2HPCR-P2A
|
This study |
N/A |
Mouse: PGK12.1: HT-CBX2SRR-S2A
|
This study |
N/A |
Mouse: PGK12.1: HT-CBX2SRR-S2E
|
This study |
N/A |
Mouse: PGK12.1: HT-CBX4 |
Zhen et al., 2016 |
N/A |
Mouse: PGK12.1: HT-CBX6 |
Zhen et al., 2016 |
N/A |
Mouse: PGK12.1: HT-CBX7 |
Zhen et al., 2016 |
N/A |
Mouse: PGK12.1: HT-CBX8 |
Zhen et al., 2016 |
N/A |
Mouse: PGK12.1: HT-MEL18 |
This study |
N/A |
Mouse: PGK12.1: HT-NLS |
Zhen et al., 2016 |
N/A |
Mouse: PGK12.1: HT-PHC1 |
This study |
N/A |
Mouse: PGK12.1: HT-RING1B |
This study |
N/A |
Mouse: Mel18−/−/Bmi1−/−
|
Elderkin et al., 2007 |
N/A |
Mouse: PGK12.1 |
Penny et al., 1996 |
N/A |
Mouse: Ring1a−/−/Ring1bfl/fl; Rosa26::CreERT2 |
Endoh et al., 2008 |
N/A |
Oligonucleotides |
|
|
Major Satellite DNA: |
IDT |
N/A |
5′-GGACCTGGAATATGGCG |
|
|
AGAAAACTGAAAATCACGG |
|
|
AAAATGAGAAATACACACTTTAG-3′ |
|
|
AT-mutated satellite DNA: |
IDT |
N/A |
5′-GGACCTGGAGTCTGGCG |
|
|
AGAGAGCTGAGACTCACGG |
|
|
ACACTGAGAGACACACACCTGAG-3′ |
|
|
Recombinant DNA |
|
|
Plasmid: pGEX-6P-1-GST-CBX2AT-P2A-FLAG |
This study |
N/A |
Plasmid: pGEX-6P-1-GST-CBX2-FLAG |
Tatavosian et al., 2019 |
N/A |
Plasmid: pMD2.G |
Zhen et al., 2016 |
N/A |
Plasmid: pSPAX2 |
Zhen et al., 2016 |
N/A |
Plasmid: pTRIPZ (M1)-CERULEAN-MEL18 |
Zhen et al., 2016 |
N/A |
Plasmid: pTRIPZ (M1)-CERULEAN-PHC1 |
Zhen et al., 2016 |
N/A |
Plasmid: pTRIPZ (M1)-HT-CBX2 |
Zhen et al., 2016 |
N/A |
Plasmid: pTRIPZ (M1)-HT-CBX21−192
|
This study |
N/A |
Plasmid: pTRIPZ (M1)-HT-CBX21−281
|
This study |
N/A |
Plasmid: pTRIPZ (M1)-HT-CBX21−498
|
This study |
N/A |
Plasmid: pTRIPZ (M1)-HT-CBX265−532
|
This study |
N/A |
Plasmid: pTRIPZ (M1)-HT-CBX289−532
|
This study |
N/A |
Plasmid: pTRIPZ (M1)-HT-CBX2ATLm
|
This study |
N/A |
Plasmid: pTRIPZ (M1)-HT-CBX2ATL-P2A
|
This study |
N/A |
Plasmid: pTRIPZ (M1)-HT-CBX2ATm
|
This study |
N/A |
Plasmid: pTRIPZ (M1)-HT-CBX2AT-P2A
|
This study |
N/A |
Plasmid: pTRIPZ (M1)-HT-CBX2CA-P2A
|
This study |
N/A |
Plasmid: pTRIPZ (M1)-HT-CBX2cd
|
This study |
N/A |
Plasmid: pTRIPZ (M1)-HT-CBX2hncr-P2A
|
This study |
N/A |
Plasmid: pTRIPZ (M1)-HT-CBX2HPCR-P2A
|
This study |
N/A |
Plasmid: pTRIPZ (M1)-HT-CBX2SRR-S2A
|
This study |
N/A |
Plasmid: pTRIPZ (M1)-HT-CBX2SRR-S2E
|
This study |
N/A |
Plasmid: pTRIPZ (M1)-HT-MEL18 |
This study |
N/A |
Plasmid: pTRIPZ (M1)-HT-PHC1 |
This study |
N/A |
Plasmid: pTRIPZ (M1)-HT-RING1B |
This study |
N/A |
Plasmid: pTRIPZ (M1)-YFP-RING1B |
Zhen et al., 2014 |
N/A |
Protein: pGEX-6P-1-GST-CBX2-FLAG |
This study |
N/A |
Protein: pGEX-6P-1-GST-CBX2AT-P2A-FLAG |
This study |
N/A |
Software and Algorithms |
|
|
Adobe Illustrator |
Adobe Inc |
N/A |
Adobe Photoshop |
Adobe Inc |
N/A |
ImageJ |
NIH |
https://imagej.nih.gov/ |
MATLAB |
MathWorks |
N/A |
OriginLab |
OriginLab Corporation |
N/A |
SlideBook 6.0 Software |
3i |
N/A |
Spot-On |
Hansen et al., 2018 |
N/A |
U-track |
Jaqaman et al., 2008 |
https://github.com/DanuserLab/u-track |
Other |
|
|
Alpha Pan-Apochromat 100 × /1.40 Oil immersion Objective |
Zeiss, Germany |
N/A |
Alpha Pan-Apochromat 100 × /1.46 Oil immersion Objective |
Zeiss, Germany |
N/A |
Axio Observer D1 Microscope |
Zeiss, Germany |
N/A |
Brightline® single-band laser filter set (Semrock; excitation filter: FF02–482/18–25, emission filter: FF01–525/45–25, and dichroic mirror: Di02-R488–25 × 36) |
Semrock |
N/A |
Brightline® single-band laser filter set (Semrock; excitation filter: FF01–561/14, emission filter: FF01–609/54, and dichroic mirror: Di02-R561–25 × 36) |
Semrock |
N/A |
Brightline® single-band laser filter set (Semrock; excitation filter: BLP01–635R-25, emission filter: FF01–640/14–25, and dichroic mirror: Di02-R635–25 × 36) |
Semrock |
N/A |
ChemiDoc XRS+ System |
Bio-Rad |
N/A |
Evolve 512 × 512 EMCCD camera |
Photometrics; Tuscon, AZ |
Cat#1708265 |
Single Channel Temperature Controller |
Warner Instruments |
Cat#TC-324 |
Solid-State LaserStack |
3i |
N/A |
Sonicator |
Vibra-cell |
VCX130 |