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. 2020 Oct 15;11:576673. doi: 10.3389/fmicb.2020.576673

Table 2.

Primers used to amplify genes to determine STs by multilocus sequence typing (MLST).

Gene Sequence 5'-3' Amplified size (bp)
cpn60 F: TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG ACT GTA CTT GCT CAA GC
R: GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GTT CAG CGA TGA TAA GAA GTG G
480 Diancourt et al. (2010)
fusA F: TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG ACA ATT ACC TCT GCT GCA ACA
R: GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA TCA TCG CAG GTT TAC GTG CT
633 This study
gltA F: TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG AGA TGT ATT GGC CTC AGG TCA CTT
R: GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACG GGT TTA CTT TGT AGT CAC GGT CTG
545 Diancourt et al. (2010)
pyrG F: TCG TCG GCA GCG TCA GTA GTG TAT AAG AGA CAG GGT GTT GTT TCA TCA CTA GGW AAA GG
R: GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GAT AAA TGG TAA AGA YTC GAT RTC ACC
434 Diancourt et al. (2010)
recA F: TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG CCT GAA TCT TCY GGT AAA AC
R: GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GGT TTC TGG GCT GCC AAA CAT TAC
425 Diancourt et al. (2010)
rplB F: TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG GTA GAG CGT ATT GAA TAC GAT CCT AAC C
R: GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GCA CCA CCA CCR TGY GGG TGA TC
472 Diancourt et al. (2010)
rpoB F: TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG GGC TTC TGA AGT ACG TGA CGT
R: GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACG TCA CGT GCA ACG TTC GCT T
502 Diancourt et al. (2010)

Citrate synthase (gltA); DNA gyrase subunit B (gyrB); glucose dehydrogenase B (gdhB); homologous recombination factor (recA); 60-kDa chaperonin (cpn60); glucose-6 phosphate isomerase (gpi); and RNA polymerase sigma factor (rpoD).