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. 2020 Oct 15;11:576673. doi: 10.3389/fmicb.2020.576673

Table 3.

Primers used to amplify CRISPR-Cas systems.

Gene Sequence 5'-3' Reference
aye-cris F: CCGTAGTTGAATCAACACGTA
R: TTTGATTGGGTAAAATGCCAAA
Hauck et al. (2012)
aye-cas1 F: TCAAGCTGCGATGCGAATGT
R: ATCCGGGCAAATTGAAACGC
Hauck et al. (2012)
ab-cris F: AGTCCCAGAGTTTTGACCCA
R: TTGGATTGGGTCATCATTGGT
Karah et al. (2015)

aye-cris: CRISPR-AYE system, aye-cas1: cas1 gene, ab-cris: CRISPR-Cas I-Fb system.