Antibody
|
Dilution
|
Company
|
Catalase; rabbit IgG |
1:1000 |
Cell Signaling Technology, Danvers, MA, USA |
nitro-tyrosine; mouse IgG2b |
1:1000 |
Abcam, Cambridge, UK |
SOD1; rabbit IgG |
1:10,000 |
Abcam, Cambridge, UK |
SOD2; rabbit IgG |
1:1000 |
Cell Signaling Technology, Danvers, MA, USA |
GAPDH; rabbit IgG |
1:10,000 |
Abcam, Cambridge, UK |
β-actin; mouse IgG |
1:10,000 |
Sigma-Aldrich, St. Louis, MO, USA |
Primer
|
Target
|
Forward/Reverse Primers (5′-3′)
|
SOD1 |
AGGCCCCTTAACTCATCT/CTACAGGTACTTTAAAGCAACTCT |
SOD2 |
GCACTAGCAGCATGTTGAGC/GCGTTGATGTGAGGTTCCAG |
Catalase |
TTTCCCAGGAAGATCCTGAC/ACCTTGGTGAGATCGAATGG |
YWHAZ |
GTCTGTAACTGAGCAAGGAGC/CTCTGCTTGTGAAGCATTGGG |