Table 1.
Information of Primer Pairs Used for RT-PCR to Validate the RNA-Seq Results.
| Primers * | Nucleotide Sequence | Expected Size |
|---|---|---|
| PVY_diag_F | ATGGCAAATGACACAATCGATGCAG | 804 bp |
| PVY_diag_R | TCACATGTTCTTAACTCCAAGTAGAGTATG | |
| CMV_diag_F | ATGGACAAATCTGAATCAACCAGTG | 754 bp |
| CMV_diag_R | GACTGGGAGCACTCCAGATG | |
| ToMV_diag_F | CGAGAGGGGCAACAAACAT | 317 bp |
| ToMV_diag_R | ACCTGTCTCCATCTCTTTGG | |
| AYVCNV_diag_F | CTGATGTGCCCAAAGGTTGT | 406 bp |
| AYVCNV_diag_R | GCCTGCTCCTTAGACGCATA | |
| TYLCKaV_diag_F | TGCCGAAGCGTTCAATAGAT | 733 bp |
| TYLCKaV_diag_R | TGTTGCATACACAGGATTAGAGG | |
| CaCV_diag_F | AAGACCTCGAAAGAGGCAAA | 703 bp |
| CaCV_diag_R | CTTCGGAGGCAAACTATTGG | |
| TNRV_diag_F | TTGCTAGCTGGAGGAGAAGC | 786 bp |
| TNRV_diag_R | TCCTCTCCTAGTTGGCTTGC | |
| PCV2_diag_F | TTCAATCGACGGTTTCACAA | 792 bp |
| PCV2_diag_R | CCTTGACTTGAGGTCGTGGT | |
| STV_diag_F | CAAAGGGAAGACTGCTGAGG | 808 bp |
| STV_diag_R | AGCCTCTCCATCGGGATTAT | |
| ChiVMV_diag_F | GCGTAAAAGGCGAAGACTCA | 782 bp |
| ChiVMV_diag_R | GTGCCGTTCAGTGTCCTCTT | |
| PMMoV_diag_F | ATGGCTTACACAGTTTCCAGTGCCAA | 474 bp |
| PMMoV_diag_R | TTAAGGAGTTGTAGCCCAGGTGAGTCC | |
| TMGMV_diag_F | ATGCCTTATACAATCAACTCTCCG | 480 bp |
| TMGMV_diag_R | CTAAGTAGCCGGAGTTGTGGTC | |
| PeVYV_diag_F | ATGAATACGGGAGGGGTTAGG | 621 bp |
| PeVYV_diag_R | CTATTTCGGGTTGTGCAATTGC | |
| LaYVV_diag_F | ATGTCGAAGCGACCTGCAGATAT | 741 bp |
| LaYVV_diag_R | GATTTTCAGAGTAGCATACACGGGA | |
| CLVd_diag_F | CGGAACTAAACTCGTGGTTCCTG | 370 bp |
| CLVd_diag_R | AGGAACCTACTGCGGTTCCA | |
| PCFVd_diag_F | CCGGATTCTTCTAAGGGTGCCT | 317 bp |
| PCFVd_diag_R | AGATCCTCTCGGGTCCCGG | |
| ToLCVV_diag_F | GCGTTAATGCGTCCCATAAT | 528 bp |
| ToLCVV_diag_R | GCATTAAAGTCGTGGGCAAT | |
| TYLCVNV_diag_F | AGAAACGCCAAGTCTGAGGA | 314 bp |
| TYLCVNV_diag_R | GTTCGGAGACGGAGAGTTGA |
* F and R indicate forward and reverse primer, respectively.