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. 2020 Oct 20;11(10):1229. doi: 10.3390/genes11101229

Figure 7.

Figure 7

Scheme of the lithocholic acid bioconversion by N. simplex VKM Ac-2033D. Substances: II-Lithocholic acid, XVII-Lithocholanoyl-CoA, XVIII-3-Oxo-5β-cholan-24-oyl-CoA, XIX-3-Oxo-chol-4-en-24-oyl-CoA, X -3-Oxo-5α-cholan-24-oyl-CoA, XXI-3-Oxo-5α-chol-1-en-24-oyl-CoA, XXII-3-Oxo-chola-1,4-dien-24-oyl-CoA, XXIII-9α-Hydroxy-3-oxo-chola-1,4-dien-24-oyl-CoA, XXIV-3-Hydroxy-9-oxo-9,10-seco-chola-1,3,5(10)-trien-24-oyl-CoA, XXV-4α-(2-Carboxyethyl)-5-oxo-7aβ,γ-dimethyl-3aα-hexahydroindane-1β-butyroyl-CoA. Biochemical reactions: 1—Ligation with CoA-SH,2—3α-Hydroxyl group dehydrogenation,3—3-Oxo-5β-cholan-24-oic acid 4(5)-dehydrogenation,4—3-Oxo-4-ene-steroids 4(5α)-reduction,5—3-Oxo-5α- or 3-oxo-4(5)-ene-cholan-24-oic acids 1(2)-dehydrogenation,6—3-oxo-1(2)-ene-cholan-24-oic acid 4(5)-dehydrogenation,7—9α-Hydroxylation. Locus tags in N. simplex are predicted, bold locus tags—genes that induced only by LCA.