. 2020 Oct 20;11(10):1229. doi: 10.3390/genes11101229

Table 3.

Genes involved in the initial steps of steroid oxidation in N. simplex.

Step	Gene Name	Annotation	Locus Tag	Cluster	Regulon	LCA	Chol	References
1	cho	cholesterol oxidase	KR76_09550		KstR	+	+	[39]
	ksdI, ksi	∆⁵-3-ketosteroid isomerase	KR76_23530		KstR		+	[40]
	ksdI, ksi	∆⁵-3-ketosteroid isomerase	KR76_24125		KstR	+	+	[40]
2	kstD3, tesH	∆¹-3-ketosteroid dehydrogenase with preference for the 1,2-saturated steroid substrates	KR76_14500	A	KstR	+	+	[41,42,43]
3, 4	cyp125	cytochrome P450 125	KR76_14335	A	KstR	+	+	[44]
6	hsd4A	17β-hydroxysteroid dehydrogenase or β-hydroxyacyl-CoA dehydrogenase	KR76_12260	B	KstR	+		[45]
7	kshA	3-ketosteroid 9α-hydroxylase, oxygenase subunit	KR76_14170	A	KstR	+	+	[46]
7	kshB	3-ketosteroid 9α-hydroxylase, reductase subunit	KR76_14160	A	KstR			[46]

Step: number of oxidation step from Figure 6. Gene name: name of gene homolog in Mycobacterium tuberculosis H37Rv or in Rhodococcus jostii RHA1. Annotation: function of enzyme. Locus_tag: locus_tag of ortholog in N. simplex VKM Ac-2033D genome that is a candidate gene for this function. Cluster: cluster of genes to which the gene is assigned (see Figure 3). Regulon: repressor whose binding site is predicted in the promoter of the gene operon. LCA: gene is induced by LCA (+). Chol: gene is induced by cholesterol (+). References: reference source where information about the gene function was taken.