FIG 2.

VU0026921 induces a metal toxicity transcriptional response. RNA sequencing was performed on mid-exponential S. aureus Newman cultures exposed to vehicle, 1 mM MnCl₂, 100 μM ‘921, or 100 μM ‘921 + 1 mM MnCl₂ for 30 min. (A) Multidimensional scaling visualization of RNA sequencing biological replicates. The x and y axes are dimensionless units. The closeness of symbols to each other on the 2-dimensional plot represents the relatedness of the transcriptional profiles of the represented data sets. (B) Categories of genes whose transcription was significantly different between 100 μM ‘921 treatment alone and cotreatment with 1 mM MnCl₂. (C) Fold change (log₂) of transcript abundance for all S. aureus genes following treatment with VU0026921 compared to vehicle-treated controls. (D) Fold change (log₂) of transcript abundance for all S. aureus genes following treatment with VU0026921 and 1 mM MnCl₂ compared to ‘921 alone. For panels C and D, genes involved in metal homeostasis and reactive oxygen species (ROS) detoxification are highlighted. Dashed lines indicate genes with greater than 2-fold change and a false-discovery rate of less than 0.01.