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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Methods Mol Biol. 2021;2191:309–321. doi: 10.1007/978-1-0716-0830-2_18

Figure 2. Example of real time PCR results produced by Transnetyx®.

Figure 2.

Chat-1 WT, and ROSA WT columns are the results using probes testing for the wild type sequence. The Chat-1 TG and YFP columns are the results of the probes testing for the mutant sequence of Cre and EYFP. “+,” and ”-” indicate expressing and non-expressing respectively. The “-” for the WTs and “+” Chat-1 TG and YFP indicate homozygosity. The TH-Cre results only show whether the animal is expressing Cre and does not indicate zygosity.