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. Author manuscript; available in PMC: 2020 Oct 29.
Published in final edited form as: Stem Cells. 2013 Oct;31(10):2095–2103. doi: 10.1002/stem.1478

Figure 3.

Figure 3.

The PI3K/AKT signaling pathway was required for hepatocyte differentiation. (A): Immunoblot analysis of the indicated proteins in definitive endoderm (DE) cells treated with the indicated growth factors for 15 minutes. (B, C): Differentiated cells were treated with or without LY294002 (10 μM) from day 8 to 24. The expression of the indicated genes was analyzed by qRT-PCR. *, p<.002 versus control in (B); *, p<.02 versus control in (C). (D): Immunoblot analysis of the phosphorylated AKT, HNF4α, and HNF1α in the DE cells treated with or without LY294002 (10 μM) for 24 hours. Abbreviations: HGF, hepatocyte growth factor.