Figure 7.

Level amount of CDK2 and Cyclin D1 in MCF7 cells treated with either HAA₂₀₂₀ (500, 2500, 5000 nM), dinaciclib (5, 25, 50 nM), or their combinations for 24 h. (A–C): immunoblots of CDK2, cyclin D1, and GAPDH, respectively. (D): Data is represented as mean ±SD (n = 2, two independent experiments). Statistical differences of the relative protein levels normalized to GAPDH were assessed by one-way Anova with the Tukey’s post-hoc multiple comparison test. p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***) were taken as significant. Image J software was used for densitometry.