Table 3.
IC50 values (72 h, nM), combination index, and selectivity index of HAA2020, tamoxifen, dinaciclib, and their combinations.
| Drug(s) nM | MCF7 | MRC5 | ||||
|---|---|---|---|---|---|---|
| HAA2020 | Tamoxifen | Dinaciclib | IC50 | *CI | IC50 | **SI |
| 125 | 480 ± 30 | 15221 ± 169 | 32 | |||
| 250 | ||||||
| 500 | - | |||||
| 2500 | ||||||
| 5000 | ||||||
| 187.5 | 738 ± 54 | 18450 ± 213 | 25 | |||
| 375 | ||||||
| 750 | - | |||||
| 3750 | ||||||
| 7500 | ||||||
| 1.25 | 4 ± 0 | 250 ± 14 | 62 | |||
| 2.5 | ||||||
| 5 | - | |||||
| 25 | ||||||
| 50 | ||||||
| HAA2020: Tam (1:1.5) | 207 ± 50 | 4558 ± 150 | 22 | |||
| 500 | 750 | 0.964 | ||||
| 2500 | 3750 | 0.823 | ||||
| 5000 | 7500 | 0.811 | ||||
| HAA2020: Din (100:1) | 1 ± 0 | 105 ± 11 | 105 | |||
| 500 | 5 | 0.463 | ||||
| 2500 | 25 | 0.301 | ||||
| 5000 | 50 | 0.260 | ||||
| Tam: Dina (150:1) | 830 ± 91 | 16600 ± 390 | 20 | |||
| 750 | 5 | >1 | ||||
| 3750 | 25 | 0.954 | ||||
| 7500 | 50 | 0.901 | ||||
| HAA2020: Tam: Dina (100:150:1) | 3 ± 0 | 153 ± 29 | 51 | |||
| 500 | 750 | 5 | 0.810 | |||
| 2500 | 3750 | 25 | 0.714 | |||
| 5000 | 7500 | 50 | 0.680 | |||
*CI: Combination index at Fa = 0.9. **SI: selectivity index = IC50 value against normal MRC-5 cells/IC50 value against MCF7. IC50 values shown in mean ±SD (n = 3). Experiments were repeated three times.