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. 2020 Oct 21;21(20):7786. doi: 10.3390/ijms21207786

Figure 5.

Figure 5

De novo sequencing of compound 1 from fraction F11 by LC-ESI-QTOF MS/MS after reduction, alkylation and protease digestion. (A) LC-ESI-QTOF MS of compound 1 (linear positive mode). Inset illustrates the MS of m/z 1598.4596 [4+]. (B) LC-ESI-QTOF MS of compound 1 after reduction, alkylation and trypsin digestion. Inset illustrates the MS of m/z 1375.6309 [1+]. (C) LC-ESI-QTOF MS/MS of precursor ion m/z 1375.6309. The peptide sequence SPFYYNPHSHK is derived by de novo peptide sequencing. Peaks corresponding to the y and b ions from this peptide are labeled on the spectrum.