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. 2020 Oct 21;21(20):7786. doi: 10.3390/ijms21207786

Figure 8.

Figure 8

Chemical synthesis of walterospermin. (A) RP-HPLC purification of peptide fragment P1. Inset, LC-ESI-QTOF MS of m/z 1454.26 [1+]. The acetonitrile gradient is provided in dashed lines: 5 to 65% for 10 min and 65 to 100% for 2 min. (B) RP-HPLC purification of peptide fragment P2. Inset, LC-ESI-QTOF MS of m/z 1435.87 [2+]. The acetonitrile gradient as in A. (C) RP-HPLC purification of peptide fragment P3. Inset, LC-ESI-QTOF MS of m/z 1071.42 [2+]. The acetonitrile gradient as in A. (D) RP-HPLC purification of P1P2 after NCL. Inset, LC-ESI-QTOF MS of m/z 1432.85 [3+]. Acetonitrile gradient is provided in dashed lines: 5 to 65% for 10 min and 65 to 100% for 2 min. (E) RP-HPLC purification of non-oxidized walterospermin (P1P2P3). Inset, LC-ESI-QTOF MS of m/z 1600.99 [4+]. The acetonitrile gradient is provided in dashed lines: 5 to 65% for 50 min and 65 to 100% for 3 min. (F) RP-HPLC purification of oxidized walterospermin. Inset, LC-ESI-QTOF MS of m/z 1599.46 [4+]. The acetonitrile gradient is provided in dashed lines: 5 to 65% for 25 min and 65 to 100% for 5 min.