Skip to main content
. 2020 Oct 19;8(10):1608. doi: 10.3390/microorganisms8101608

Figure 4.

Figure 4

Determination of the enzymatic activity of recEhADH3Bb. (A). The catalytic activity of recEhADH3Bb was determined with a range of substrates (formaldehyde (50 mM), acetaldehyde (20 mM), propionaldehyde and butyraldehyde, methanol, ethanol, propanol, butanol (all 100 mM)) in the presence of 0.7 mM NADP(H) at pH 7.4. (B–D) Enzyme kinetic of recEhADH3Bb using acetaldehyde as substrate. (E,F) Enzyme kinetic of recEhADH3Bb using formaldehyde as substrate. (B,E) Time dependence of enzymatic activity; (C,F) Michaelis-Menten plot; (D,G) Lineweaver–Burk plot.