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. 2020 Oct 19;21(20):7732. doi: 10.3390/ijms21207732

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Radial view representation of an unrooted Neighbor-Joining tree depicting the phylogenetic relationship among (Gα) protein subunits of Gs, Gi/Go, Gq/G11 and G12/G13 classes of representative vertebrate homologs. saGαi1 and saGαi2 AA sequences cluster in one-to-one ortholog relationships within the corresponding Gαi monophyletic clade, suggesting saGαi1-2 duplicated origin from ancient vertebrate whole genome duplications. Numbers above nodes indicate bootstrap values (based on 1000 replicates) that support the respective branch. The scale (lower left corner) indicates the mean number of AA substitutions per site.