Figure 4.

Evaluation of pGL3-NFAT-luc construct responsiveness to 10-fold serial dilutions to PMA (A) and ionomycin (B) in transiently transfected HEK293 cells. RLU%: Relative Luminescence Unit percentage. Evaluation of L-AA responses was based on the RLU mean ± SEM of four independent determinations normalized to the mean response of the same transfections (n = 4) stimulated with assay medium (Dulbecco’s Modified Eagle Medium (DMEM) + 1% fetal bovine serum (FBS)). BL (Basal Levels) = 100% RLU. Different lowercase letters (a,b) on top of bars indicate significant differences (p < 0.05) between concentrations, assessed by one-way ANOVA followed by Tukey’s Multiple Comparison Test (GraphPad Prism version 5.00 Software, La Jolla, CA, USA).