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Screening of putative stable pGL3-NFAT-luc-HEK293 clones by response to PMA (n = 4) at 10-fold serial dilutions tested over three concentrations (10−8, 10−7 and 10−6). Evaluation of PMA responses was based on the RLU mean ± SEM of four independent determinations normalized to the mean response of the same transfections stimulated with assay medium (DMEM +1% FBS). BL (Basal Levels) = 100% RLU. Clone 3 (Cl.3) was the most responsive to PMA-luminescence induction.
