Table 3. Calibration and Validation Results for the Adenosine and Cordycepin Concentrations in C. militaris Fruiting Bodies Prepared in Four Different Formsa.
| calibration set |
validation set |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| bioactive compound | sample preparations | preprocessing methods | PLS factors | Rc2 | SEC | Rp2 | RMSEP | SEP | bias | RPD |
| adenosine (mg kg–1) | fresh | no preprocess | 8 | 0.98 | 19.61 | 0.85 | 48.40 | 49.61 | –1.13 | 2.66 |
| SNV | 10 | 0.98 | 21.15 | 0.85 | 49.10 | 49.86 | –5.74 | 2.64 | ||
| COE | 9 | 0.98 | 16.94 | 0.91 | 38.10 | 38.42 | –7.55 | 3.45 | ||
| fresh chopped | no preprocess | 7 | 0.94 | 32.63 | 0.95 | 26.90 | 27.50 | –8.57 | 5.04 | |
| SNV | 8 | 0.98 | 18.81 | 0.93 | 33.99 | 34.43 | 3.71 | 3.83 | ||
| COE | 8 | 0.98 | 19.19 | 0.94 | 30.80 | 30.36 | –10.40 | 4.44 | ||
| dried powder | no preprocess | 9 | 0.96 | 31.88 | 0.92 | 35.2 | 36.12 | 0.12 | 3.66 | |
| SNV | 7 | 0.92 | 38.44 | 0.93 | 32.60 | 28.75 | –10.30 | 4.17 | ||
| COE | 9 | 0.95 | 33.01 | 0.92 | 34.80 | 35.56 | –3.50 | 3.72 | ||
| dried crude extract | no preprocess | 6 | 0.86 | 74.44 | 0.86 | 46.60 | 47.33 | –3.82 | 2.77 | |
| SNV | 7 | 0.88 | 48.50 | 0.88 | 43.50 | 44.40 | 3.35 | 2.97 | ||
| COE | 5 | 0.76 | 65.24 | 0.84 | 50.20 | 51.56 | –1.43 | 2.56 | ||
| cordycepin (mg kg–1) | fresh | no preprocess | 10 | 0.99 | 275.23 | 0.95 | 502.00 | 512.87 | –76.1 | 4.96 |
| SNV | 9 | 0.99 | 331.66 | 0.97 | 386.00 | 386.83 | 86.30 | 6.54 | ||
| COE | 7 | 0.97 | 528.49 | 0.95 | 533.00 | 534.42 | –115.00 | 4.74 | ||
| fresh chopped | no preprocess | 7 | 0.99 | 308.62 | 0.97 | 365.00 | 356.39 | –36.60 | 6.78 | |
| SNV | 8 | 0.98 | 455.58 | 0.97 | 397.00 | 378.85 | –147.00 | 6.68 | ||
| COE | 8 | 0.99 | 248.66 | 0.98 | 277.00 | 283.69 | –1.05 | 8.90 | ||
| dried powder | no preprocess | 7 | 0.99 | 181.44 | 0.99 | 226.00 | 230.31 | –28.10 | 11.00 | |
| SNV | 10 | 0.99 | 158.23 | 0.99 | 218.00 | 223.99 | –22.50 | 11.30 | ||
| COE | 10 | 0.99 | 188.42 | 0.99 | 196.00 | 198.78 | –29.30 | 12.70 | ||
| dried crude extract | no preprocess | 9 | 0.99 | 185.30 | 0.98 | 308.00 | 312.89 | –69.90 | 8.21 | |
| SNV | 6 | 0.99 | 303.46 | 0.98 | 339.00 | 313.92 | –66.90 | 7.40 | ||
| COE | 9 | 0.99 | 142.68 | 0.98 | 337.00 | 335.33 | –85.60 | 7.54 | ||
a
Rc2: determination coefficient in calibration, SEC: standard error of calibration, Rp2: determination coefficient in prediction, RMSEP: root mean square error of prediction, SEP: standard error of prediction, and RPD: ratio of prediction to deviation (RPD = SD/SEP).