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. 2020 Oct 22:1–25. doi: 10.1080/07391102.2020.1835726

Table 8.

In silico prediction of physicochemical and pharmacokinetics properties of top binding alkaloids.

(a) Lipinski filter analysis
Lipinski filters
Cryptospirolepine
10-Hydroxyusambarensine
Cryptoquindoline
Isocryptolepine
Strychnopentamine
Molecular weight (g/mol) 504.58 448.56 448.52 232.28 459.75
Num. heavy atoms 39 34 35 18 41
Num. rotatable bonds 2 2 1 0 4
Num. H-bond acceptors 1 3 2 1 4
Hydrogen bond donor 1 3 0 0 3
cLogP 3.75 3.31 4.02 4.02 3.51
Molar refractivity 161.96 142.46 145.65 76.01 180.14
Lipinski violation
1
0
0
0
1
(b) Admet SAR
Absorption (Probability)
Blood-Brain Barrier BBB+ (0.99) BBB+ (0.83) BBB+ (0.95) BBB+ (0.97) BBB+ (0.71)
Human intestinal absorption HIA+ (0.97) HIA+ (0.98) HIA+ (0.99) HIA+ (0.99) HIA+ (0.98)
Bioavailability score 0.55 0.55 0.55 0.55 0.55
Caco-2 permeability Caco2+ (0.74) Caco2+ (0.53) Caco2+ (0.76) Caco2+ (0.67) Caco2+ (0.53)
P-glycoprotein substrate Nonsubstrate (0.50) Substrate (0.91) Noninhibitor (0.69) Noninhibitor (0.74) Noninhibitor (0.87)
P-glycoprotein inhibitor Noninhibitor (0.84) Noninhibitor (0.60) Noninhibitor (0.72) Noninhibitor (0.74) Noninhibitor (0.54)
Renal organic cation transporter Noninhibitor (0.70) Inhibitor (0.80) Noninhibitor (0.67) Noninhibitor (0.54) Noninhibitor (0.70)
Distribution (Probability)
Subcellular localization Mitochondria (0.70) Mitochondria (0.65) Mitochondria (0.55) Mitochondria (0.76) Mitochondria (0.57)
Metabolism
CYP450 Substrate Substrate (0.69) Substrate (0.53) Substrate (0.86) Substrate (0.65) Nonsubstrate (0.79)
  Noninhibitor (0.76) Noninhibitor (0.83) Noninhibitor (0.86) Noninhibitor (0.81) Inhibitor (0.54)
Toxicity
AMES toxicity Non AMES toxic (0.61) Non AMES toxic (0.75) AMES toxic (0.89) AMES toxic (0.80) Non AMES toxic (0.68)
Carcinogens Noncarcinogens (0.94) Noncarcinogens (0.97) Noncarcinogens (0.92) Noncarcinogens (0.96) Noncarcinogens (0.95)
Acute oral toxicity III (0.50) III (0.51) III (0.67) III (0.72) III (0.56)
Rat acute toxicity LD50 (mol/kg) 2.4992 2.7896 2.4420 2.3770 2.7631
Aqueous solubility (LogS) –3.5692 –2.7626 –3.1120 –3.21 –2.6192
Pharmacokinetics
GI absorption Low High Low High High
Log Kp (skin permeation) (cm/s) –4.82 –5.70 –3.97 –5.38 –5.82