Fig. 7.

Gap19 regulates the physical association between astrocytic Cx43 and YAP in the cytoplasm after hemin stimulation. a, b Cx43 was overexpressed by lentiviral transfection, and the levels of cytoplasmic/nuclear YAP were measured by WB in hemin-stimulated astrocytes. Quantitative analysis showed that Lv-Cx43 transfection decreased nuclear YAP translocation after hemin stimulation. However, Cx43 overexpression had no effects on YAP nuclear translocation after Gap19 treatment. c Coimmunoprecipitation experiments showing that Cx43 interacted with Gap19 but not YAP in hemin-stimulated astrocytes. d, e Coimmunoprecipitation experiments showing that YAP interacted with Cx43 and that Gap19 interfered with the binding of astrocytic Cx43 to YAP in the cytoplasm after hemin stimulation. f Representative images of the Duolink in situ PLA showing that there was a direct interaction between YAP and Cx43, that the effect was enhanced after Cx43 overexpression by Lv-Cx43, and that the interaction was weakened after Gap19 treatment. The bars represent the SEM of the data from 3 samples per group. *P < 0.05, **P < 0.01 compared to the hemin group. #P < 0.05, ##P < 0.01 compared with the Gap19 + hemin group