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. 2020 Oct 27;4(20):5297–5310. doi: 10.1182/bloodadvances.2020002297

Figure 5.

Figure 5.

Belinostat decreases STAT1 and IRF1 expression and significantly enhances the antilymphoma activity of Reolysin. (A) Belinostat treatment decreases STAT1 and IRF1 expression. Parental TCL cells were treated with 5 and 10 μM belinostat for 24 hours. STAT1 and IRF1 expression were determined by immunoblotting. (B-C) Reolysin enhances the activity of belinostat in both parental and belinostat-resistant TCL cells. Cells were treated with 0.25 μM belinostat and 45 or 90 PFUs Reolysin per cell, respectively, for Karpas-299 and HuT-78 and the combination for 72 hours (B) or 48 hours (C). Cell viability was determined by MTT assay and apoptosis was measured by PI-FACS analysis. Data are shown as mean ± SD (n = 3). *P < .05 indicates a significant difference compared with Control or **P < .05 compared with either monotherapy.