Figure 4.

The different strains have similar ICaL and ICaL restitution. (A) I-V relationship was similar in WT, PLNKO, and S2814D myocytes and was enhanced with increasing extracellular Ca²⁺. (B) Original traces of the protocol used to assess ICaL restitution kinetics. (C and D) Experimental and model results, respectively, of ICaL restitution curves showing that ICaL inactivation recovery is enhanced by increasing extracellular Ca²⁺. (E and F) Experimental and model results, respectively, of ICaL restitution curves. Ablation of PLN or increased RYR2 activity by RYR2 Ser2814 pseudophosphorylation did not significantly alter ICaL restitution. Experimental data were expressed as mean ± SEM. Numbers inside bars represent the number of animals used; numbers within parentheses inside bars represent the number of cells. When several cells per animal were used, results of the number of cells in each animal were averaged. The numbers used for statistical analysis correspond to the number of animals. P values are denoted above the bars.