Fig. 3.

Oligodendrocyte/Myelin impairments in adult Trem2^Y38C/Y38C and Trem2^−/− mice. a-c Mbp, Mobp and Olig2 transcript levels were determined by quantitative-PCR. a Mbp transcripts were reduced in both Trem2^Y38C/Y38C and Trem2^−/− cortical lysates and b-c Mobp and Olig2 mRNA reduced significantly in Trem2^−/− cortical lysates. d Western blot analysis and e Quantification of myelin proteins CNPase and MBP. CNPase levels were reduced significantly in Trem2^−/− mice and Mbp levels were significantly reduced in both Trem2^Y38C/Y38C and Trem2^−/− mice. a-e Data represented as mean ± SEM. N = 4 (2 males, 2 females); One-way ANOVA with Tukey’s post hoc test; *P < 0.05, **P < 0.01, ***P < 0.001