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. 2020 Sep 30;219(11):e202006054. doi: 10.1083/jcb.202006054

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© 2020 Zhang et al.

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Figure S3. — Cry2WT-mCherry-Tau 151–254 (CWT 151–254)/EB1-EGFP bound along the MT lattice of MT bundles upon light activation. Time-lapse of EB1-EGFP (green) when overexpressed with Cry2WT-mCherry-Tau 151–254 (CWT 151–254, red) in SH SY5Y cells activated by shallow blue light (488 nm, 5% power). Note that besides binding MT +TIPs and moving as comets, upon light activation, some CWT 151–254/EB1-EGFP extended the binding along the MT lattice and MT bundles (following the blue arrows in the time-lapse) while the cell at the bottom, which also has expression of EB1-EGFP but no CWT 151–254, does not show shift of EB1 localization from MT +TIPS, remaining as a comet (white arrows). Montage of two fluorescent channels is presented in the order from left, the mCherry channel, the GFP channel separate, and merged. Scale bar, 10 µm.