Skip to main content
. 2020 Sep 30;219(11):e202006054. doi: 10.1083/jcb.202006054

Table 2. Primers used for generating the listed plasmids.

Plasmids Forward primer Reverse primer
Cry2 G490E 5′-GAA​GCA​CAG​ATC​ATG​ATC​GGA​G-3′ 5′-ACG​GGT​TCT​TGA​AAT​AGC​TTT​AGC-3′
Cry2WT-mCherry-Tau 1-375 5′-TGA​GGA​TCC​ACC​GGA​TCT​AGA​TAA​C-3′ 5′-CTT​GTG​GGT​TTC​AAT​CTT​TTT​ATT​TCC​TCC​G-3′
Cry2WT-mCherry-Tau 1-343 5′-TGA​GGA​TCC​ACC​GGA​TCT​AGA​TAA​C-3′ 5′-CTT​CTC​AGA​TTT​TAC​TTC​CAC​CTG​G-3′
Cry2WT-mCherry-Tau 1-311 5′-TGA​GGA​TCC​ACC​GGA​TCT​AGA​TAA​C-3′ 5′-TTT​GTA​GAC​TAT​TTG​CAC​ACT​GCC-3′
Cry2WT-mCherry-Tau 1-281 5′-TGA​GGA​TCC​ACC​GGA​TCT​AGA​TAA​C-3′ 5′-CTT​CTT​ATT​AAT​TAT​CTG​CAC​CTT​CCC​G-3′
Cry2WT-mCherry-Tau 1-254 5′-TGA​GGA​TCC​ACC​GGA​TCT​AGA​TAA​C-3′ 5′-CTT​CAG​GTC​TGG​CAT​GGG-3′
Cry2WT-mCherry-Tau 244-375 5′-CAG​ACA​GCC​CCC​GTG​CCC-3′ 5′-CAT​TGA​ATT​CGA​AGC​TTG​AGC​TCG-3′
Cry2WT-mCherry-Tau 151-375 5′-ATCGCCACACCGCG-3′ 5′-CAT​TGA​ATT​CGA​AGC​TTG​AGC​TCG-3′
Cry2WT-mCherry-Tau 151-254 5′-TGA​GGA​TCC​ACC​GGA​TCT​AGA​TAA​C-3′ 5′-CTT​CAG​GTC​TGG​CAT​GGG-3′
Cry2WT-mCherry-Tau 151-254 T231E 5′-CCG​TCT​TCC​GCC​AAG​AGC​C-3′ 5′-CGA​CTT​GGG​TGG​CTC​ACG​GAC​CAC​TGC​CAC​CTT​C-3′
Cry2WT-mCherry-Tau 151-254 S235E 5′-CCG​TCT​TCC​GCC​AAG​AGC​C-3′ 5′-CTC​CTT​GGG​TGG​AGT​ACG​GAC​CAC​TGC​CAC​CTT​C-3′
Cry2WT-mCherry-Tau 151-254 T231E/S235E 5′-CCG​TCT​TCC​GCC​AAG​AGC​C-3′ 5′-CTC​CTT​GGG​TGG​CTC​ACG​GAC​CAC​TGC​CAC​CTT​C-3′
Cry2WT-mCherry-Tau 151-254 T212E/S214E/T217E 5′-CCC​ACC​CGG​GAG​CCC​AAG​AAG-3′ 5′-TGG​TTC​TGG​AAG​TTC​CGG​TTC​GCG​GGA​GCG​GCT​GCC​G-3′
Cry2WT-mCherry-Tau 151-254 S202D/T205D/S208D 5′-GAC​CCC​GGC​GAC​CGC​TCC​CGC​ACC​CCG-3′ 5′-GCC​TGG​ATC​GCC​GGG​GCT​GCT​GCT​GTA​GCC-3′
Cry2WT-mCherry-Tau 151-254 S202A/T205A/S208A 5′-GCT​CCC​GGC​GCC​CGC​TCC​CGC​ACC​CCG-3′ 5′-GCC​TGG​TGC​GCC​GGG​GCT​GCT​GTA​GCC-3′
Cry2WT-mCherry-Tau 151-254 S202A/T205A/S208A/T212A/S214A 5′-CTT​CCA​ACC​CCA​CCC​ACC​CG-3′ 5′-TGC​CGG​TGC​GCG​GGA​GCG​TGC​GCC​GGG​TGC​GCC​TGG​TGC​GCC​GGG​GCT​GCT​GTA​GCC-3′
Cry2WT-mCherry-Tau 151-254 I171A/P172A 5′-GCA​AAA​ACC​CCG​CCC​GC-3′ 5′-TGC​TGC​CCT​GGT​GGC​GTT​GGC​CT-3′