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. 2020 Sep 30;219(11):e202006094. doi: 10.1083/jcb.202006094

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© 2020 Brandt et al.

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Figure 3. — The SYP-1 T452A mutation partially rescues targeting of PLK-2 to pairing centers in hal-2 mutants. (A) Immunofluorescence images of leptotene/zygotene nuclei from wild-type, hal-2, and hal-2; syp-1^T452A mutants showing PLK-2::mRuby (white or red), HTP-3 (blue), and HIM-8 (green). Scale bar, 5 µm. (B) Graph showing the percentage of nuclei with paired HIM-8 foci from the indicated genotypes. To score pairing of the X chromosome pairing center, the germline was divided into five zones as shown in the diagram. Zone 2 contains leptotene/zygotene nuclei (n = 154 for wild type; n = 170 for hal-2; n = 159 for hal-2; syp-1^T452A), and zone 3 contains nuclei in early pachytene (n = 170 for wild type; n = 211 for hal-2; n = 144 for hal-2; syp-1^T452A). ***, P < 0.0001; ns, not significant (P > 0.05) by χ² analysis. (C) Immunofluorescence images of pachytene nuclei from wild-type, hal-2, and hal-2; syp-1^T452A mutants showing the staining for HTP-3, HTP-1/2, and HIM-3. Scale bar, 5 µm.