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. 2020 Sep 30;219(11):e202006094. doi: 10.1083/jcb.202006094

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© 2020 Brandt et al.

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Figure 7. — CDK-1 is required for chromosome remodeling after crossover formation. (A) Immunofluorescence images of late-pachytene nuclei from control and CDK-1–depleted animals showing DNA (white), PLK-2 (red), and COSA-1 (green) staining. Scale bar, 5 µm. (B) Graph showing the quantification of COSA-1 number per nucleus in wild-type (n = 50) and CDK-1–depleted animals (n = 50). Mean ± SD is shown. ns, not significant (P > 0.05) by two-tailed Mann-Whitney test. (C) Composite immunofluorescence images of diakinesis nuclei from control and CDK-1–depleted animals showing DNA (white), HTP-1/2 (green), and HIM-3 (red) staining. Scale bar, 10 µm. (D) Zoomed-in images of a diplotene nucleus from control and CDK-1–depleted germlines as indicated in C. Scale bars, 5 µm. (E) Zoomed-in images of an individual chromosome in diakinesis from control and CDK-1–depleted germlines as indicated in C. Scale bar, 1 µm.