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. 2020 Sep 30;219(11):e202006094. doi: 10.1083/jcb.202006094

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© 2020 Brandt et al.

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Figure S1. — Phospho-specificity of the SYP-1 pT452 antibody used in this study, and the analysis of SYP-1 T452 phosphorylation and PLK-2 localization in chk-2 mutants. (A) In vitro kinase assays using human CDK-1/cyclin A to phosphorylate MBP–SYP-1 with or without the T452A mutation. Western blot using antibodies against SYP-1 and phosphorylated SYP-1 at T452 is shown. (B) Composite immunofluorescence images of a whole gonad dissected from syp-1^T452A mutants showing DNA, SYP-1, and SYP-1 pT452 staining. Scale bar, 50 µm. (C) Composite immunofluorescence images of a whole CHK-2–depleted gonad showing DNA (white), SYP-1 (red), SYP-1 pT452 (green), and PLK-2::3Flag staining. Scale bar, 50 µm.