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. 2020 Sep 30;219(11):e202006094. doi: 10.1083/jcb.202006094

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© 2020 Brandt et al.

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Figure S3. — Enrichment of SYP-1 phosphorylation on the short arm requires PLK-2 kinase activity and crossover formation. (A and B) Zoomed-in images of nuclei from leptotene/zygotene (A) and early pachytene (B) showing PLK-2::3Flag (red) and phospho-HIM-8/ZIMs (green) staining. Scale bars, 5 µm. (C) Composite immunofluorescence images of a whole gonad dissected from the plk-2^K65M (kinase dead) homozygote animal showing DNA (blue), COSA-1 (yellow), and SYP-1 pT452 staining (white). Scale bar, 50 µm. (D) Late-pachytene nuclei from wild-type and cosa-1(tm3298) mutants were stained for DNA, SYP-1 (red), SYP-1 pT452 (green), and PLK-2 (magenta). Scale bar, 5 µm. Diagrams illustrating the results are shown on the right.