Figure S2.
Quantification of liposome recruitment assays. (A) The ratio of Sec31 to Sec23 was quantified from multiple independent liposome recruitment experiments (see Fig. 2 B) using the indicated proteins, revealing a slight deficit in recruitment of Sec31C. (B) The ratio of Sec31 to Sec23 was quantified from multiple independent experiments (see Fig. 3 C) using the indicated proteins, revealing no difference in recruitment. (C) Ratios of Sec23 to Sar1 (left values) and Sec31 to Sec23 (right values) were calculated from multiple independent experiments (see Fig. 4 D) revealing that Sec23-Δgel was recruited like WT protein to Sar1 but was impaired in its ability to recruit Sec31. In each case, error bars depict standard deviation, and the statistical test was a one-way ANOVA with Dunnett’s multiple comparisons test. If no P value is given, then there was no difference.