Figure 3.

Alteration of platelet activation markers, β-thromboglobulin (A) and P-selectin (B), after blood recirculation in the Impella 5.5 and Centrimag circulatory loops. β-Thromboglobulin level in blood plasma was assessed by ELISA. P-selectin exposure on platelet surface was detected by flow cytometry and reported as MFI of marker-positive platelets. Data of three experiments are reported as mean ± SD, one-way repeated measures ANOVA with Dunnett’s multiple comparison test: **^,##p < 0.01 as compared with nonsheared control “0 Still” for Impella 5.5 and Centrimag, respectively. No statistically significant difference was found between devices for β-thromboglobulin and P-selectin (two-tailed paired t-test, p > 0.05). ANOVA, analysis of variance; ELISA, enzyme-linked immunosorbent assay.