Fig. 2.

BRD4 knockdown inhibits GH3 cells proliferation in vitro. (A, B) Knockdown efficiency of the BRD4-specific siRNAs and their impact on BRD4 relevant oncogene c-Myc expression in GH3 cells, tested by real-time PCR and western blotting. (C) Normalized proliferation curve of siCtrl and siBRD4#3-treated GH3 cells. (D) Macroscopic images and quantified colonies formed by GH3 cells. All the experiments were repeated 3 times with consistent results. **P < 0.01; ***P < 0.001. Bar represents mean ± SD.