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. 2020 Oct 27;61(12):25. doi: 10.1167/iovs.61.12.25

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Figure 3. — Augmented expression of HSP70 in RHOT17M mice. (A) Immunoblots confirm the expression of TgCrx-Hspa1a-Flag transgene and elevated total HSP70 protein level in RHOT17M mice at 3 months of age. (B) Marginally increased ONL thickness was observed in RHOT17M; TgHspa1a+ (n = 8) mice at 5 months of age when compared with RHOT17M; TgHspa1a- (n = 6) littermates. (C) Representative pictures of central and peripheral region of H&E stained retinal cross-sections from RHOT17M;TgHspa1a- and RHOT17M;TgHspa1a+ mice at 5 months. (D) Plots of scotopic and photopic ERG amplitudes of RHOT17M; TgHspa1a- (n = 16 and 11, respectively) and RHOT17M; TgHspa1a+ (n = 16 and 9, respectively) mice at 3 and 5 months. Scale bar: 25 μm for all panels. Error bar: SEM. Student's t-test, *P < 0.05.