Figure 5.

Augmented expression of HSP70 in rd10 mice. (A) Immunoblots confirm the expression of TgCrx-Hspa1a-Flag transgene and elevated total HSP70 protein level in rd10 mice at P20. (B) Decreased ONL thickness, more pronounced in the retinal periphery, was observed in rd10; TgHspa1a+ (n = 5) mice at P30 when compared with rd10; TgHspa1a- (n = 5) littermates. (C) Representative picture of central and peripheral region of H&E stained retinal cross-sections from rd10;TgHspa1a- and rd10;TgHspa1a+ mice at P30. (D) Plots of scotopic and photopic ERG amplitudes of rd10; TgHspa1a- (n = 5 and 10) and rd10; TgHspa1a+ (n = 10 for both ages) mice at P20 and P30, respectively. Scale bar: 25 μm for all panels. Error bar: SEM. Student's t-test, *P < 0.05, **P < 0.01, ***P < 0.001.