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CLSM images of NCI-H460 cells incubated with various nanoparticles containing FAM-siRNA (green) after 4 h (A), 12 h (B) and 24 h (C). Lyso Tracker Red (red) was used to label the endosome/lysosome and Hoechst 33258 (blue) to label nucleus. (D) The ratio of green fluorescence to red fluorescence was quantified with Image-Pro Plus.
