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. 2020 Oct 23;27(1):1397–1411. doi: 10.1080/10717544.2020.1827085

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — VEGF protein expression was quantified by the ELISA method. NCI-H460 cells were treated with different nanoparticles after incubation for 48 h. VEGF protein expression of nanoparticles without sucrose laurate (A) and with sucrose laurate (B) was detected. siNC was used as the negative siRNA control. Each bar represents the mean ± SD of at least three experiments. Cell viability of nanoparticles without sucrose laurate (C) and with sucrose laurate (D) was detected by CCK-8 assay. NCI-H460 cells (5000 cells/well) were seeded in a 96-well plate and treated with different formulations for 72 h. Results are presented as the mean ± SD (n = 3). Cell apoptosis of nanoparticles without sucrose laurate (E) and with sucrose laurate (F) was observed by Hoechst 33258 assay. Scale bar = 50 μm.