Figure 1.

Panel of reporter flaviviruses. A. Scheme of NanoLuc insertion in the flavivirus genome. The duplicated capsid portion varies from 25 to 38 amino acids, depending on the virus. The white beginning of the capsid gene indicates the codon scrambling to reduce homology with the duplicated portion. B. Virus, corresponding number of capsid amino acids duplicated, and representative IFA images from Day 1 to 3 post electroporation. The pan-flavivirus envelope antibody 4G2 was used to probe for virus and cells were counterstained with DAPI. C. Focus-forming assay after 4-day infection on Vero cells. D. Genetic stability results after two independent series of ten passages in Vero cells. Viral RNAs from each passage were used as templates in an RT-PCR reaction with primers that spanned the 5’ UTR to the capsid. Full-length band sizes are as follows ZIKV: 1,162 bp; DENV1: 1,086 bp; DENV2: 1,128 bp; DENV3: 1,126 bp; DENV4: 1,131 bp; YFV: 1,282 bp; JEV: 1,360 bp.