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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Mol Reprod Dev. 2020 Jul 31;87(8):880–886. doi: 10.1002/mrd.23403

FIGURE 2.

FIGURE 2

Regional calcium rise induced by microneedles in wildtype oocytes and calcium waves in kug mutant oocytes. (a) Representative images showing calcium waves propagating from the ends of kug mutant near-spherical shaped mature oocytes (n = 15). Arrowheads: wavefront of the calcium wave. (b–d) Representative images showing regional calcium rises that do not propagate into waves induced by microneedle pressing at (b) anterior (n = 7), (c) waist (n = 4), and (d) posterior (n = 4) regions of wildtype mature oocytes. Arrowheads: pressing site of microneedles. The background level of calcium varies among mature oocytes (Hu & Wolfner, 2019); scale bar = 100 μm